African Journal of Biotechnology Vol. 6 (18), pp. 2130-2134, 19 tribe 2007 Available online at http://www.academicjournals.org/AJB ISSN 16845315 © 2007 Academic Journals Full length research Paper Biodiesel generation from insincere yeast Rhodotorula glutinis with xylose ingest capacity Chuan-chao Dai*, Jie Tao, Feng Xie, Yi-jun Dai and Mo Zhao Jiangsu Key Laboratory for Biological smorgasbord and Biotechnology, College of Life Science, Nanjing universal University, Nanjing, Jiangsu Province, 210046, China. Accepted 15 August, 2007 This study explored a strategy to diversify agricultural and forestry residues into microbial lipoid, which could be advance transformed into biodiesel. Among the 250 yeast strains screened for xylose assimilating capacity, eight oleaginous yeasts were selected by Sudan Black B test. The lipid content of these 8 strains was determined by soxhlet beginning method. One strain (T216) was give to produce lipids up to 36.6%, and it was identified as Rhodotorula glutinis. The best zymosis conditions were obtained as follows: glucose as carbon seed 100 g/L; yeast extract and peptone as nitrogen sources at, respectively, 8 and 3 g/L; initial pH of 5.0; inoculation volume of 5%; temperature at 28oC, shaking repair of clxxx r/min, cultivated for 96 h.

Under these conditions, R. glutinis amass lipids up to 49.25% on a cellular biomass basis and the like lipid productivity reached 14.66 g/L. Experiments with a 5-L bioreactor under the optimal finale conditions showed that R. glutinis accumulated lipids up to 60.69%, resulting in 23.41 g/ L in lipid productivity. More encouraging re! sults were observed for the lipid deed with alternative carbon sources. Corn stalk and Populus euramevicana leaves hydrolysate could be used to substitute glucose. Chemical analysis indicated that biodiesel obtained by transesterification be in possession of similar composition to that from ve possessable oil, one of the widely...If you want to get a full essay, order it on our website:
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